抗胰岛素单克隆抗体的研究

Studies on the Monoclonal Antibody against Insulin

用人工合成的人胰岛素原类似物基因大肠杆菌表达产物免疫 BALB/ c小鼠制备免疫脾细胞 ,与小鼠骨髓瘤 SP2 / 0细胞系融合 ,建立了 7株能分泌抗胰岛素单克隆抗体 (Mc Ab)的杂交瘤细胞系。间接 EL ISA和免疫印迹试验结果表明 ,该单克隆抗体对人。

Lines of hybridomas secreting monoclonal antibodies (McAb) against insulin were established by the fusion of mouse myeloma cells SP2/O and spleen cells from BALB/c mouse immunized with the products expressed in Escherichia coli Containing the synthesized human proinsulin analog gene Determination for the McAb with indirect ELISA and western blotting indicated that the McAb Could specifically react with insulin from human, bovine and swine pancreas