血红素氧化酶诱导机制的初步研究

Primary Studies on Mechanism of Induction of Heme Oxygenase

对血红素氧化酶的诱导在整体水平上表现为血红素氧化速率提高了 8倍 ;在培养的肝组织水平 ,诱导后单位重量组织的酶活力单位也提高了 8- 10倍 ;在酶分子水平上 ,酶活力表现与诱导剂的存在与否无关 ,排除了诱导剂作为激活剂或辅因子的作用。实验结果表明该酶的诱导能被放线菌素 D和嘌呤霉素抑制并表现出不同的抑制效应 ,但与

The induction effects at the whole bodies were presented in rate of heme oxidation enhanced 8 folds, and the increase 8～10 folds in enzyme activity per gram of liver weght reflected the induction at the tissue level The possibility of inducer playing a role of enzyme activator was eliminated because of no relationship between the existance or no of inducer and enzyme activity at the level of enzyme molecule The induction was found not to be related to DNA replication but was inhibited by actinomycin D and puromycin and produced different inhibitory effects