气相色谱-原子发射光谱联用仪对安眠镇静类和局麻类药物的非同一标样定量分析

QUANTITATIVE ANALYSIS OF SOPORIFIC SEDATIVE DRUGS AND LOCAL ANESTHETIC DRUGS WITH UNIDENTICAL STANDARD SUBSTANCE BY GC AED

针对药物组成繁多、结构复杂、标准品较难收集的特点，本文利用气相色谱原子发射光谱联用仪（ＧＣＡＥＤ）检测元素的特性，研究了使用非同一标样对安眠镇静类和局麻类药物进行定量分析的可能性，优化了实验条件，测定了９种安眠镇静药和８种局麻药的相对响应因子。计算了它们的相对标准偏差：１０２１％。结果显示：在优化的分析条件下，选择与待测物相对响应因子相近的物质作为标样。

According to the principle of GC AED(gas chromatography atomic emission detector),the possibility of quantitative determination of samples by different substances as internal standard was studied. The element C was determined at wavelength of 496 nm. The relative response factors of 17 soporific sedative and local anesthetic drugs were analyzed with an internal standard of proadifen hydrochloride under optimized analytical conditions. Oven temp.: 45℃(0 5 min)—35℃·min -1 —120℃—15℃·min -1 —220℃—5℃·min -1 —250℃; Cool on column injector temp: 60℃—50℃·min -1 —250℃(0 2 min); inject volume: 1 μl; column head pressure: 145 kPa. The results show that the element C(496) in identical or unidentical substances has similar relative response factor. The RSD is 10 21%, which means that a sample can be quantitatively determined by a different substance when the analysis condition and the selected internal standard are suitable. An amobarbital sample of 109 0 μg·ml -1 was quantitatively determined by using methylphenobarbitone, pramoxine, lidocaine and itself as internal standard. The results are 117 6, 115 6, 110 0 and 102 14 μg·ml -1 , respectively. They can be quantitatively determined by each other.