黑曲霉果胶裂解酶和聚半乳糖醛酸酶合成的发酵调控

Fermentation Conditons of Aspergillus niger for Production of Pectin Lyase and Polygalacturonase

通过对黑曲霉(Aspergillus niger)wz 003液态发酵过程的调控,制备了高活性的果胶裂解酶,并讨论了发酵过程中聚半乳糖醛酸酶活力的变化情况。实验表明,发酵产酶培养基组成为(g/L):麸皮60,玉米粉40,酵母膏20,胡萝卜12,CaCO_3 10。培养条件为:初始pH 6.5,30℃,接种量8%,培养2 d。此时果胶裂解酶活力为375.3 U/mL,为基础条件下的6.225倍,而聚半乳糖醛酸酶的合成得到了有效控制,酶活仅为29.4 U/mL。改变发酵条件,有利于聚半乳糖醛酸酶的发酵生产,此时酶活力为基础条件下的5.05倍。

Fermentation conditions for Aspergillus niger wz003 for production of high active pectin lyase was investigated in this paper. The effect of polygalacturonase activity was also tested. The experimental result indicated： the optimal fermentation medium was （g/L） ：wheat bran 60, cornmeal 40,yeast extract 20, carrot12, CaCO310. After inoculation with 8%（v/w, 106entries/mL） A. niger wz003 , the medium was cultivated at 30℃ for 2d with original pH 6.5, the pectin lyase activity could reach 375.3 U/mL,increased to 6. 225 times of that cultivated under the basic conditions, and the synthesized of polygalacturonase was effectively controlled. The enzyme activity was 29.4 U/mL. When the fermentation conditions change, the polygalacturonase activity could reach 5.05 times of which cultivated under basic conditions.