酶法制备汉麻籽蛋白抗氧化肽

Preparation of Antioxidant Peptides from Hempseed Protein by Enzymatic Hydrolysis

采用不同蛋白酶酶解汉麻籽蛋白,确定Alcalase 2.4L碱性蛋白酶是酶解汉麻籽蛋白制备抗氧化肽的优良酶源。通过单因素和响应面回归分析,得到Alcalase 2.4L碱性蛋白酶酶解汉麻籽蛋白的优化条件为:底物浓度50 mg/mL、水解时间2 h、温度50℃、加酶量2.2%、pH 9.4。优化酶解条件下,水解度约为20%,10 mg/ mL酶解产物的DPPH自由基清除率为82.65%,显示出较好的抗氧化活性。

Hempseed protein was hydrolyzed with four different enzymes. It was found that Alcalase 2.4L alkaline protease had the best scavenging effect to DPPH · . Using response surface regression analysis, we achieved the optimum enzyme hydrolysis conditions of Alcalase 2.4L alkaline protease as： substrate （[S]）50 mg/mL, time2 h, temperature （T） 50℃, enzyme： substrate ratio （[E]/[S]）2.2%, and pH 9. 4. The obtained degree of hydrolylsis （DH） was about 20 %, the antioxidant activity of 10 mg/mL hydrolysate was about 82.65 %, which showed high antioxidant activity.