潜力工业催化剂——白地霉脂肪酶

Versatile Industrial Biocatalyst-Geotrichum candidum Lipase

白地霉脂肪酶因具有独特的底物特异性,在油脂改性等领域有着广泛应用。文中概述了白地霉脂肪酶的发酵生产、分离纯化、克隆与表达、底物特异性分子机理及应用等,并展望其未来发展方向,为该工业催化剂的研发与广泛应用提供参考。

Geotrichum candidum lipase with chemo-specificity are unique in catalyzing the hydrolysis of triacylglycerols and it reverses the reaction in non-aqueous solvents, which occupy a place of prominence among biocatalysts owing to their novel and multifold applications in enzymatic restructuring of oils and fats, resolution of racemic mixtures. Fermentation and production, purification, gene cloning and expressing, mechanism of substrate specificity for Geotrichum candidum lipase were reviewed in this paper, which could be utilized as consultation references for research and application of this enzyme biocatalyst.