p53基因第249密码子突变与肝细胞癌癌栓形成有关

RELATIONSHIP BETWEEN THE MUTATION OF p53 GENE AT CODON 249 AND THE FORMATION OF TUMOR EMBOLIZATION IN HUMAN HEPATOCELLULAR CARCINOMA

目的:以 p53基因第249密码子突变为指标,研究与肝细胞癌高侵袭性有关的分子机理。方法:联合应用 PCR与限制性内切酶反应方法,研究48例癌细胞及其癌周肝组织中 p53基因第249密码子突变情况及其与肝细胞癌高侵袭性的关系。结果:48例癌组织中有20例(41.7%)发现有 p53基因第249密码子突变,而48例癌周肝组织均未发现有 p53基因突变。p53基因第249密码子突变率在有癌栓组显著高于无癌栓组(68.8%对28.1%,P 值<0.01)。而在不同病灶数目组、不同大小肝癌组及不同包膜状况肝癌组中,p53基因第249密码子突变率差别没有显著意义。结论:p53基因第249密码子突变与肝细胞癌癌栓形成有关。

Objective:p53 gene was used as an index to study the molecular pathogenesis association with the high invasiveness of human hepatocellular carcinoma (HCC).Methods:The mutation at codon 249 of p53 gene in 48 HCC samples and their surrounding liver tissues and its relationship with the high invasiveness of HCC were studied by combined polymerase chain reaction (PCR) with the HaeⅢ enzyme restriction analysis.Results:20 of 48 HCC samples (41.7%) had p53 mutation at codon 249,whereas none of the surrounding liver tissues had p53 mutation.The mutational rate of p53 gene at codon 249 in HCC samples with tumor embolus was higher than that in HCC samples without tumor embolus (68.8% vs 28.1%,P<0.01).However,significant difference on mutational rate of p53 gene at codon 249 was not found between HCC with multiple and single nodule,small and large HCC as well as between HCC with and without capsule.Conclusion:The mutational rate of p53 gene at codon 249 may be related to the forma- tion of tumor embolus in HCC.