脂肪酶催化合成拟鲸蜡油工艺

Technology for Lipase-Catalyzed Spermaceti Analogue

研究了无溶剂体系下脂肪酶催化长链混合脂肪酸和鲸蜡醇酯化合成一种液态蜡酯混合物的过程,该液态蜡酯混合物能作为鲸蜡油和霍霍巴油的替代品,对三种商业酶和自制固定化脂肪酶进行了筛选,并采用响应面分析方法对酯化反应进行优化,以酸的转化率为响应值,对温度、酶量、底物摩尔比、时间这四个因素的重要性做了适当评价,并给出了拟和良好、回归显著、可靠性较好的经验性模型方程。优化条件为:反应温度44℃,固定化酶用量0.2 g/g酸,底物酸醇摩尔比1:1,反应时间11h,酸的转化率可达93%,水解活力为150 IU/g的固定化酶的半衰期约为100 h。

The synthesis reaction of a spermaceti analogue by lipase - catalyzed esterification of mixed long- chain fatty acids with long - chain alcohol in non - solvent system was discussed. The lipase was chosen based on screening from lipases of commercial sources as well as that produced in the laboratory. In order to get high conversion yield of the product, some important parameters such as temperature, enzyme amount, molar ratio of substrate, reaction time were optimized by the response surface methodology. The obtained optimum 1 ： 1, enzyme dosage 0.2 g/g acid, and reaction time 11 h parameters are temperature 44 ℃, molar ratio of acid to alcohol At above conditions, the conversion rate is 93 % and the half- life of the immobilized lipase with hydrolysis activity of 150 IU/g can be longer than 100 h.