核因子κB在前置胎盘患者胎盘组织中的表达

The Expression of the Nuclear Factor-κB in the Pacental Tissue of Patients with Placenta Praevia

目的研究核因子κB在前置胎盘患者胎盘血管平滑肌及内皮细胞中表达的变化及其在前置胎盘发病过程中的作用。方法经HE染色后镜下观察胎盘组织及血管的病理变化。免疫组织化学方法及Western blot检测前置胎盘患者胎盘组织中NF-κB的表达。结果HE染色显示前置胎盘胎盘绒毛血管合体膜增厚,胎盘细小血管平滑肌细胞增生,纤维素样坏死明显多于正常妊娠组织;免疫组织化学显示前置胎盘患者胎盘血管平滑肌细胞及内皮细胞NF-κB胞浆、胞核染色较正常妊娠明显增强;Western blot显示前置胎盘患者胎盘组织胞浆、胞核NF-κB含量、胞核/总NF-κB含量比值明显高于正常妊娠组织。结论NF-κB的激活及增加可能为前置胎盘发病机制的重要信息途径之一。

Objective To study the changes of nuclear factor-κB （NF-κB） activity in the placenta of normotensive and pregnancy induced placenta praevia. Methods The NF-κB expression and activity in placental tissue were determined by immunohistochemistry, image analysis and western blot. Results The fibrinoid necrosis and other pathological changes of the placeta vessel in placenta praevia were significantly higher than those in normal group. The expression of NF-κB in placental blood vessel of placenta praevia was greatly stronger than that in normal group tissue. The amount of total, cytosoic and nuclei NF-κB and the ratio of cytosoic to total NF-κB amount in placenta praevia were higher than those in normal group tissue. Conclusion The NF-κB signal pathway may regulate the expression of placenta and arterial collagenin in placenta praevia, which may play an important role in the pathogenesis of placenta praevia.