成年斑马鱼牙齿发育的形态学观察

Study on the morphology of zebrafish tooth in adult stage

目的:观察成年斑马鱼牙齿的生长部位和形态。方法:以成年斑马鱼(鱼龄>3个月)为研究对象,在体视显微镜下,解剖成年斑马鱼腮弓,对其牙齿进行活体观察,并做石蜡切片和HE染色观察。结果:成年斑马鱼牙齿位于第五腮弓上,每条腮弓上牙齿数量不等。牙齿外形呈三角形,为端生牙,无牙根。三角形牙齿的底部附着于腮弓上,三角形牙齿的尖即为牙尖,朝向内侧咽部,左右牙尖相对。HE染色显示成年斑马鱼的牙齿为钙化结节样物质,类似人牙齿的牙本质结构。结论:对斑马鱼牙齿组织形态和发育过程的进一步研究将有利于寻找到一种良好的研究牙齿组织发育的模式动物。

Objective：To research the location and morphology of zebrafish tooth in adult stage. Methods：The zebrafish （ages greater than 3 months） were included in this experiment. The pharyngeal arch skeleton was dissected from the adult zebrafish. The morphology of tooth was observed under the stereomicroscope or under the light microscope by para^num section and HE staining. Results：There are several teeth located on the paired fifth pharyngeal arch. The teeth are acrodont that have no dental root. The shape of the teeth is triangulum whose tip is the teeth cusp. The left and right teeth cusp oppose to each other forward to the pharyngeal cavity. By HE staining,the histological character of teeth are calcified tissue as same as the dentin of human tooth. Conclusion：The future research about the morphology and development process of the zebrafish tooth will help to find a kind of optimal model animal to the tooth develpoment research.