雷公藤甲素对类风湿关节炎滑膜成纤维细胞系MH7A中VEGF、MMP-9水平变化的影响

Effect of triptolide on the changes of VEGF and MMP-9 levels in the rheumatoid arthritis fibroblast-like synoviocyte line,MH7A

目的:研究雷公藤甲素对类风湿关节炎(RA)滑膜成纤维细胞系MH7A培养上清液中血管内皮生长因子(VEGF)、基质金属蛋白酶-9(MMP-9)水平变化的影响。方法:肿瘤坏死因子-α(TNF-α)刺激RA滑膜成纤维细胞系MH7A后,以不同剂量TP干预,酶联免疫吸附试验(ELISA)检测细胞培养液上清中VEGF、MMP-9水平的变化。结果:TNF-α刺激后,细胞培养上清液中VEGF及MMP-9分泌不同程度增加。TP各剂量组(1、5、25、75μg/ml)在不同时间点(24、48、72h)均可以明显下调培养上清液中MMP-9和VEGF的水平,与地塞米松对照组比有显著差异。结论:雷公藤甲素能抑制血管新生有关细胞因子VEGF、MMP-9的表达。

Objective：To study the effect of triptolide on the expression of VEGF and MMP-9 in the rheumatoid arthritis fibroblast-like synoviocyte line,MH7A. Methods：Rheumatoid arthritis fibroblast-llke synoviocyte line,MH7A were incubated for 24 hours with 20 ng/ ml of recombinant human TNF-α then were treated with Triptolide of different concentration（ 1μg/ml,5 μg/ml, 25 μg/ml and 75 μg/ml）. Levels of vascular endothelial growth factor（VEGF） and matrix metallproteinases（MMP-9）were measured in cell supernatants by Enzyme- Linked ImmunoSorbent Assay （ELISA）. Results：After TNFct stimulation, VEGF and MMP-9 production was highly increased in the supernatants. Triptolide caused a dose-dependent decrease in VEGF and MMP-9. The inhibited effect of Triptolide on the expression of VEGF and MMP-9 is stronger than that of Dexamethasone. Conclusion： Triptolide can inhibit the regulative factor of angiogenesis VEGF and MMP-9 expression.