过敏性紫癜患儿血液脂氧素A_4的变化及其临床意义

Significance of detecting blood lipoxin A_4 in children with Henoch-Schonlein purpura

目的:检测过敏性紫癜患儿在不同发病阶段的血清脂氧素A4(LXA4)的变化,并观察与白介素(IL)-5、IL-6、IL-8和C反应蛋白(CRP)的变化的关系,以探讨LXA4、IL-5、IL-6、IL-8和CRP在过敏性紫癜发病机制中的作用。方法:采集27例过敏性紫癜患儿急性期和恢复早期血液,以及26例正常儿童血液,应用酶联免疫吸附试验(ELISA)检测血清中LXA4、IL-5、IL-6、IL-8和CRP含量。结果:过敏性紫癜患儿的急性期和恢复早期血液LXA4、IL-5、IL-6、IL-8和CRP含量明显高于正常组的含量(P<0.01);恢复早期患儿LXA4含量明显高于急性期(P<0.01)。急性期血液IL-5、IL-6、IL-8和CRP含量明显高于恢复早期血液含量(P<0.01)。LXA4与IL-5、IL-8呈正相关(P<0.05);LXA4与IL-6、CRP无显著相关性(P>0.05)。IL-5、IL-6、IL-8和CRP水平各组之间存在正相关性(P<0.01)。11例重型患儿的急性期、恢复早期LXA4均低于16例轻型患儿(P<0.05)。结论:过敏性紫癜患儿的急性期血液LXA4、IL-5、IL-6、IL-8和CRP含量升高;恢复早期IL-5、IL-6、IL-8和CRP含量有所下降,而LXA4含量更高;提示IL-5、IL-6、IL-8可能参与过敏性紫癜的病变,而LXA4可能有抗炎作用。重型患儿的LXA4低于轻型患儿,提示体内抗炎物质LXA4不足可能与病情加重有关。

Objective：To investigate the serum levels of lipoxin A4 （LXA4） in Children with Henoch-Schonlein purpura （HSP） in different phase, and explore the correlation of LXA4 with interleukin （IL）-5,IL-6, IL-8 and C RP in the pathogenesis of HSP. Meth- ods：The blood levels of LXA4,IL-5 ,IL-6,IL-8 and CRP in 26 normal children and 27 children with HSP in acute phase and earlier recovery phase were detected by ELISA,respectively. Results：The blood levels of LXA4, IL-5,IL-6,IL-8 and CRP in children with HSP in acute and earlier recovery phase were significantly higher than those in normal group（P 〈 0.01 ）,and the blood level of LXA4 in children with HSP in earlier recovery phase was significandy higher than that in the acute phase （P 〈 0.01）. However,the blood levels of IL-5, IL-6,IL-8 and CRP in children with HSP in acute phase were significantly higher than those in earlier recovery phase （P 〈 0.01 ）. There were positive correlations between LXA4 and IL-5 or IL-8. Meanwhile,there were no positive correlations between LXA4 and IL-6, LXA4 and CRP. There were positive correlations among IL-5, IL-6, IL-8 and CRP. The LXA4 levels of severe patients in acute and earlier recovery phase were lower than that in mild cases. Conclusion：These data suggest that IL-5 ,IL-6 and IL-8 may play an injurious role, and LXA4 may play a protective role in the pathogenesis of HSP. The insufficiency of LXA4,an anti-inflamma- tory mediator in human body, may be a reason for the patients with HSP whose illness become more serious.