全反式维甲酸在慢性肾衰大鼠体内的变化及其意义

Change of all-trans retinoic acid content in chronic renal failure rats and the meaning

目的:外源性全反式维甲酸(all-trans retinoic acid,atRA)能延缓慢性肾衰的病理进程,因此我们在本研究中检测了慢性肾衰时内源性atRA的水平是如何波动的。方法:建立5/6肾大部切除的大鼠肾衰模型,采用反相高效液相色谱仪(RP-HPLC)检测了5/6肾大部切除大鼠不同时间点的血、肾组织atRA的水平。结果:与假手术组(sham)比较,单纯肾大部切除组(NX)血肌酐水平自第10周起明显高于sham组(P<0.05)。NX组大鼠血浆atRA在第2周有一个明显的升高,第7周降至接近正常或正常以下;NX组肾组织atRA水平自第2周时间点有明显升高,直到第10周才降至接近正常或正常以下,同sham组比较没有差异。sham组大鼠血浆和肾组织atRA的含量比较稳定。结论:正常大鼠血浆和肾组织内有atRA存在,含量比较稳定。5/6肾大部切除后,大鼠血浆和肾组织内atRA在短期内升高,以后明显下降,在中、晚期降至正常水平。

Objective： atRA is a kind of indispensible substance in human body. In order to understand the change of all- trans retinoic acid content in chronic renal failure rats, we have designed the following study. Methods： 5/6 nephroectomized rat model was used in this trial. The 5/6 nephroectomized rats were assigned into non-intervention group （NX group, n=48） and sham group （n=30）. Eight rats in NX group and 5 in sham group were killed respectively in the beginning of the trial （week 0）, week 2, 4,7, 10 and 13. RP-HPLC was used to examine atRA level in blood and kidney at those time point. Results： In NX group the plasma atRA content presented an obvious increase in the 2nd week and it reduced to normal or below normal value. Similarly, atRA content in kidney in NX group also presented an obvious increase in the 2nd week, which was higher than that of sham group. But unlike plasma atRA, kidney atRA content did not decrease immediately and it was still higher than that in sham group in the 4th and 7th week （P〈0.05）. Until the 10th week did it re- duced to normal or below normal value. Either plasmic or renal atRA levels kept steady in sham. Conclusion：① The a-tRA contents in palsma and kidney of healthy rats are steady. ② atRA contents in rat plasma and kidney after 5/6 renal ablation would increase for a short time followed by decrease and they reduce to normal value in middle or late stage.