一种新型全白细胞分离液的研制

Research on a novel leukocytes separation liquid

目的:为了有效地分离、提取外周血全白细胞,研制一种新型的细胞分离液。方法:右旋糖苷(聚蔗糖)和泛影葡胺按不同比例混合配制成四种比重、五种渗透压的分离液。通过对50例正常外周血的分离摸索出最佳全白细胞分离液的比重和渗透压。结果:最佳全白细胞分离液的比重为1.112 g/ml,渗透压为520 mOsm/L。结论:我们自制的全白细胞分离液提取细胞浓度及细胞纯度高,细胞组分全,细胞比例正常,细胞形态及细胞活性保持良好。同时全白细胞分离液还可以进行其他细胞分离,为相关学科的研究提供了一种有效的细胞分离新方法。

Objective： In order to isolate and purify efficiently human leukocytes subpopulations from peripheral blood, we have prepared a new hquid separation. Methods： Dextran （Fieoll） and Hypaque were mixed in different proportions to four gravity and five osmotie pressure of separation liquid. By isolated 50 normal peripheral blood,we groped the best gravity and osmotic pressure. Results： Dextran（Fieoll）-Hypaque with density of 1.112 g/ml and osmolarity of 520 mOsm/L was the best results.Conclusion： White blood eells （WBC） are isolated by the novel liquid separation whose eoneentration and purity are high. It eoneluded all eomponents of WBC, and its proportion is normal, eells shape and viability remain good. At the same time, the eell liquid separation ean also isolate the other eells whieh ereate a new type of eell isolation methods for the research in many areas.