摘要
目的:观察白藜三醇(RES)对培养的豚鼠心室肌细胞L-型钙电流(ICa-L)密度的影响。方法:分离成年豚鼠心室肌细胞,采用全细胞膜片钳技术,记录不同浓度RES孵育心室肌细胞不同时间的ICa-L密度。结果:①培养72h时,RES30μmol/L和100μmol/L使ICa-L密度分别下降13%和27%,呈现浓度依赖性抑制趋势,且RES100μmol/L组与对照组比较有统计学差异(P<0.05);②RES10μmol/L,30μmol/L和100μmol/L对ICa-L密度呈现时间依赖性抑制趋势,且RES100μmol/L在24h和72h之间有统计学差异(P<0.05);结论:白藜三醇对培养豚鼠心室肌细胞L-型钙电流呈现浓度和时间依赖性抑制趋势。
Objective:To investigate the effect of resveratrol on the density of L-type calcium current. (ICa-L) in cultured guinea pig ventricular myocytes. Methods:Single cultured ventricular cells were obtained from healthy adult male guinea pig heart using enzymatic dissociation methods. The whole-cell patch clamp was used to record the ICa-L after administration of resveratrol at 24 h and 72 h with different final concentration of 0, 10,30,100 μmol/L. Results: (1)30 μmol/L and 100 μmol/L RES decreased the density of ICa-L by 13% and 27%,respectively,at 72 h. There is a significant difference between control group and RES 100 μmol/L group(P 〈 0.05); (2)RES 10 μmol/L, 30 μmol/L and 100 μmol/L have a tendency of reducing the density of ICa-L in a time-dependent manner. 100 μmol/L RES reduced ICa-L more significantly at 72 h compared with that did at 24 h (P 〈 0.05). Conclusion:Resveratrol have a tendency of inhibiting the density of L-type calcium current in cultured guinea pig ventricular myocytes at a concentration-and time-dependent manner.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2008年第8期964-967,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省高校自然科学研究项目(05KJB320085)
国家自然科学基金面上项目(04BA30470708)
关键词
白藜三醇
心室肌细胞
全细胞膜片钳
L-型钙电流密度
resveratrol
cultured ventricular myocyte
whole-cell patch clamp
L-type calcium current