高脂饮食致大鼠非酒精性脂肪肝模型的建立

Establishment of nonalcoholic fatty liver model of rat induced by high fat diet

目的:建立一种简单易行的大鼠非酒精性脂肪肝动物模型。方法:将20只SD大鼠随机分为两组,A组(对照组)给予基础饲料喂养4周,B组(模型组)给予高脂脂饲料喂养4周;4周内观察两组大鼠一般情况及体重变化,4周后取材检测比较大鼠血清谷草转氨酶(AST)、白蛋白(ALB)、甘油三酯(TG)水平,肝脏HE染色病理学检查,并计算肝指数。结果:模型组肝指数、TG明显高于对照组,AST、ALB两组无统计学差异,病理学检查证实模型组肝脏呈轻中度脂肪肝变性。结论:成功建立适用于脂肪肝供肝研究的动物模型。

Objective：To establish the nonalcoholic fatty liver model of rat induced by high fat diet. Methods：Twenty Sprague-Dawley（SD）rats were divided randomly into two groups：normal control group fed with common diet and model group fed with high fat diet for 4 weeks. The general conditions and weight changes were observed for 4 weeks. All rat were sacrificed at the end of 4 th week of experiment. Blood samples were collected for measure the alanine aminotransferase（ALT）, albumin（ALB）, triglyceride （TG）. The liver specimens were stained with HE for histology. Liver index was counted. Results：The liver index and triglyceride （TG） of model group were significantly higher than those of control group. The alanine aminotransferase （ALT） and albumin （ALB） between two groups didn＇t have significant difference. The histological observation showed mild to moderate hepatic steatosis. Conclusion：The nonalcoholic fatty liver model of rat was successfully established.