TIG2在皮肤鳞癌中的表达及意义

Alteration and significance of tazarotene induced gene-2(TIG2) in skin squamous cell carcinoma

目的:研究TIG2(tazarotene induced gene-2)在皮肤鳞癌中的作用机制。方法:用免疫组化和原位杂交的方法检测正常皮肤组织,未受累组织及鳞癌皮损中TIG2蛋白和mRNA的表达变化。结果:TIG2蛋白和mRNA表达于正常组织及未受累组织的表皮全层;在鳞癌的边缘皮损中,可见TIG2表达于角质层,棘层上中部,而不表达于棘层下部及基底层;在Ⅰ～Ⅱ期鳞癌中,TIG2可轻度表达于分化较好的角化珠周围;而在Ⅲ～Ⅳ期鳞癌中无表达。在正常组织和未受累皮肤中,TIG2的表达高于鳞癌组织中的表达(P<0.01);边缘皮损基底层中的表达低于正常组织及未受累组织基底层中的表达(P<0.01);在Ⅲ～Ⅳ期鳞癌中的表达明显低于鳞癌边缘皮损中的表达(P<0.01)。结论:TIG2可能参与鳞癌的发生和发展。

Objective：To investigate the role of tazarotene induced gene-2 （TIG2） in the pathogenesis of skin squamous cell carcinoma （SCC）. Methods：TIG2 protein and mRNA in normal skin tissues,uninvolved skin and SCC lesions were detected by immunohistochemistry and hybridization in situ. Results：TIG2 protein and mRNA were expressed in all layers of normal and uninvolved epidermis. In addition, TIG2 expression could be observed in the comeum,granular layers and the upper or middle layers of stratum spinosum of the marginal part of SCC lesions. Furthermore,TIG2 positive expression could only be observed slightly around the keratin pearls of SCC Ⅰ -Ⅱ stages,and no expression in SCC Ⅲ-Ⅳ stages. TIG2 expression is less in aggressive SCC compared with that in the normal skin and uninvolved tissues from SCC （P 〈 0.01 ）; and TIG2 expression is absent in the basal layer of marginal epidermis of SCC compared with that in normal skin and uninvolved tissues from SCC patients（P 〈 0.01 ） ;moreover,TIG2 expression is also less in SCC Ⅲ-Ⅳ than in the marginal tissues of SCC（P 〈 0.01 ）. Conclusion：TIG2 may play an important role in the pathogenesis of SCC.