高效毛细管电泳-电化学检测对外消旋氯霉素前体的拆分研究

Enantioseparation of the Prodrug of Chloramphenicol by Capillary Zone Electrophoresis with Electrochemical Detection

用毛细管电泳-电化学检测法对外消旋苏式-2-氨基-1-对硝基苯基-1，3-丙二醇（氯霉素前体）进行了拆分研究．考察了影响检测及分离效果的实验参数，同时对其拆分机制进行了初步探讨．

Chiral separation of the enantiomers of threo-2-amino-1- (4-nitrophenyl )-1, 3-propanediol, namely the prodrug of chloramphenicol (PCP), was carried out by capil1aryzone electrophoresis with electrochemical detection employing β-cyclodextrin (β-CD) as achiral additive in strong alkaline solutions. The analytes were detected by electrochemistryusing a copper disk electrode at +675 mV versus Ag/AgCl reference electrode. Both the freeenantiomers and the enantiomer-cyclodextrin inclusion complexes could be detected usingthis approach, although the complexed forms gave lower oxidation currents than the uncomplexed ones. Factors affecting the chiral CE separation of the analytes, such as concentrationof running buffer and β-CD, applied voltage, were extensively investigated. Under the optimum conditions, baseline separation of the enantiomers could be accomplished in less than l8min. In addition, an attempt was made to elucidate the pIausible mechanism 0f the chiralrecognition.