酶法提取竹节参中多糖成分的工艺研究

Study on extraction process of polysaccharide constituents from Panax japonicus by enzyme method

目的:研究酶法提取竹节参中多糖成分的工艺。方法:以多糖得率为考察指标,采用正交试验法对竹节参中多糖成分酶法的提取工艺进行优选。结果:优化后的提取工艺为pH 5.0,50℃,料液比为1:50,纤维素酶的质量分数1.2%,果胶酶的质量分数1.0%,酶解时间2h,90℃灭酶和浸提2h,多糖得率为14.3%。结论:酶法能有效提高竹节参多糖成分的得率。

Objective： To choose the optimum extraction process of polysaccharide constituents from Panax japonicus. Method ： The optimal compound enzyme extraction process is selected with the orthogonal design. The yield of polysaccharide was used to evaluate the factor levels, so as to determine the condition of the optimum extraction process. Result： The conditions of the ideal extraction process using compound enzyme method were that of the pH value was at 5.0, the extraction temperature was at 50 ℃, solvent volume was 50 times of material, cellulase quality score was 1.2%, pectinex quality score was 1.0% and the extraction time was 2 hours. At the last, the vitalitive enzyme was deactivated in the 90 ℃ water for 2 hours. Under these conditions, the yield of polysaccharide extract from P. japonicus was up to 14. 3%. Conclusion. The yield of polysaccharide extracted from P. japonicus can be efficetively increased by using compound enzyme extraction process.