绿升麻中1个新环阿屯烷型三萜皂苷

A new cycloartane triterpene saponin from rhizome of Actaea asiatica

目的:研究绿升麻中对肿瘤细胞有细胞毒活性的化学成分。方法:以硅胶柱、凝胶等多种柱色谱分离,制备高效液相色谱纯化,以各种波谱鉴定化合物结构。结果:分离得到1个环阿屯烷型三萜类化合物,鉴定其结构为(23R)-16β,23:23α,26:24α:25-triepoxy-9,19-cyclolanost-7-en-3—O-β-D-xylopyranoside。结论:化合物1是新化合物,命名为(23R)-26-deoxycimicifugoside。化合物1对宫颈癌细胞(Hela)和小鼠成纤维细胞(L929)具有一定的细胞毒作用,IC_(50)分别为72.24,55.97 mg·L^(-1)。

Objective： To isolate and elucidate the chemical constituents with the cytotoxicity activity from the rhizome of Actaea asiatica. Method： The chemical constituents were isolated by repeated column chromatography （Toyopearl HW-40C and preparative HPLC） and the structure of compound 1 was elucidated by spectral data analysis. Result： A cycloartane triterpene saponin com-pound was isolated and identified to be （23R）-16β, 23： 23α, 26： 24α： 25-triepoxy-9, 19-cyclolanost-7-en-3β-O-β-D-xylopyranoside. Conclusion： Compound 1 was a new compound and named （23R）-26-deoxycimicifugoside. The IC50 values of compound 1 for cell growth inhibition of Hela and L929 cell lines were 72.24 and 55.97 mg·L^-1, respectively.