山梗菜生物碱类化合物的ESI-MS^n研究

Studty on alkaloids in Lobelia sessilifolia by ESI-MS^n

目的:对山梗菜中生物碱类化合物进行定性分析。方法:利用固相萃取技术(SPE)、电喷雾多级串联质谱技术(ESI-MS^n)对山梗菜中生物碱类化合物进行定性分析。结果:初步确定了山梗菜中的2个生物碱结构分别是异山梗菜酮碱和8,10-二乙基山梗二酮,并根据其在正离子条件下表现出的特征质谱行为,提出了山梗菜中生物碱类化合物可能的电喷雾质谱碎裂规律。结论:固相萃取技术、电喷雾多级串联质谱技术能够快速简便地对山梗菜中生物碱类化合物进行定性分析。

Objective： To make qualitative analysis on alkaliod in Lobelia sessilifolia. Method： The ESI-MS^n method was used for qualitative analysis. Result： The structures of two alkaliods in plant extract of L. sessilifolia were successfully analyzed and identified as norlobelanine and 8, 10-dlethyllobelidione by electrospray ionization multistage mass spectrometry in positive mode. Then the fragmentation mechanisms were explored. Conclusion： SPE and ESI-MS^n could simply and rapidly provide qualitative examination of alkaliods in L. sessilifolia.