维肝力对骨髓抑制小鼠骨髓及血清FL分泌水平的影响

Effect of Weiganli on level of FL in bone marrow and serum of myelosuppressed anemic mice

目的:研究维肝力对骨髓抑制贫血小鼠骨髓及血清Flt 3配体(FL)分泌水平的影响,探讨维肝力在骨髓造血调控方面的作用。方法:制作骨髓抑制贫血小鼠模型,小鼠随机分为正常组、骨髓抑制贫血模型组、维肝力组(高、中、低剂量100,50,25 g·L^(-1));观察维肝力对骨髓抑制贫血小鼠外周血象、骨髓有核细胞(BMC)数的影响;通过酶联免疫吸附技术(ELISA)检测了骨髓抑制贫血小鼠骨髓及血清Flt3配体(Flt3 ligand,FL)的水平。结果:高剂量维肝力对粒细胞系、红细胞系、血红蛋白(Hb)及BMC均有显著的提高作用,中、低剂量维肝力对血小板促进作用更明显;维肝力组骨髓及血清FL水平均低于贫血组,其中高、中剂量组FL水平下降最为显著。结论:维肝力对放、化疗所引起的小鼠骨髓抑制能起到一定的缓解作用。

Objective： To study the effect of Weiganli on the level of FL in bone marrow and serum of myelosuppressed anemic mice, and to explore it＇s function on hematopoietic regulation. Method： Models of myelosuppressed anemic mice were induced by radiation and chemotherapeutic drug, and the mice were randomly divided into normal group, myelosuppressed anemic group,and Weiganli group （high dose 100 g·L^-1 , medium dose 50 g·L^-1 , low dose 25 g·L^-1 ）. Effect of Weiganli on the number of the peripheral blood cells and bone marrow nucleated cells （BMC） were evaluated. Effect of Weiganli on the level of FL （Flt3 ligand） was investigated by ELISA technique. Result： High dose of Weiganli could significantly increase granulocytes, erythrocytes, Hb and BMC, while both the medium dose and the low dose had more significant action in increase of platelet. The level of FL in bone marrow and serum were lower in Weiganli group than that in myelosuppressed anemic group, especially in high and medium dose group. Conclusion： The myelosuppresion of mice which induced by radiation and chemotherapeutic drug could be significantly relieved by Weiganli.