冠心Ⅱ号对大鼠缺血心肌蛋白差异表达的影响

Effects of Guanxin Ⅱ cardiac protein expression after acute myocardial ischemia on rats

目的:研究冠心Ⅱ号对大鼠缺血心肌蛋白差异表达的影响。方法:随机分为假手术组、模型组和冠心Ⅱ号给药组。假手术组、模型组用饮用水灌胃,冠心Ⅱ号给药组按10 g·kg^(-1)灌胃;每日1次,连续10 d后手术。模型组和冠心Ⅱ号给药组予结扎冠状动脉成心肌缺血动物,假手术组只穿线不结扎。手术后24 h取心肌缺血交界区组织进行蛋白双向电泳、差异表达质谱分析等检测。结果:心肌缺血后差异蛋白表达数目增加,冠心Ⅱ号干预后又有所增加,其中有效蛋白11个。缺血时上调蛋白数目多,用药后有一定减少。调节关键蛋白的意义涉及抑制细胞形态结构变化,抑制亢进的能量代谢。结论:冠心Ⅱ号从蛋白水平对大鼠缺血心肌有一定影响。今后应进一步扩大差异筛选的范围,以获得更全面的结果。

Objective： Guanxin Ⅱ is a famous modem formula of traditional Chinese medicine. Guanxin Ⅱ after myocardial infarction （MI） has been shown to have beneficial effects on cardiac anatomy and function. The purpose of this study was to examine the effects of Guanxin Ⅱ on cardiac protein expression after MI. Method ： Rats were randomized into 3 groups, sham, model and treating groups. Model and treating groups were fed with Guanxin Ⅱ and sham group was fed with water for 10 days before MI. MI operation is to ligate left coronary artery. 24 hours after MI, myocardial protein expression of junctional zone was assessed with 2D gel electrophoresis and mass spectra analysis. Result： Guanxin Ⅱ was found to be able to improve myocardial protein expression, especially 11 proteins. These proteins are mainly involved in suppressing changes of cell shape and structure and energy metabolism. Conclusion： Guanxin Ⅱ after MI affected myocardial protein expression. Further experiments of larger research extent should be done to receive more results.