黄芪甲苷的大鼠在体肠吸收动力学的研究

Study on absorption kinetics of astragalosideⅣin rats intestines

目的:研究黄芪甲苷的在体肠吸收机制。方法:采用大鼠在体单向肠灌流实验,利用HPLC法测定黄芪甲苷的含量,分别研究吸收部位、药物浓度、P-糖蛋白抑制剂对黄芪甲苷吸收的影响。结果:各肠段的吸收速率常数(K_a)和表观吸收系数(P_(app))有显著性差异(P<0.05);在20～80 mg·L^(-1)内,小肠吸收速率常数和表观吸收系数无显著性差异;P-糖蛋白抑制剂对黄芪甲苷的肠吸收影响不大。结论:黄芪甲苷的肠吸收为典型的被动扩散吸收机制。

Objective： To investigate the mechanisms of intestine absorption of astragaloside Ⅳ in rat. Method： The Ka and Papp of astragaloside Ⅳ was investigated using single-pass intestinal perfusion technique in rats. HPLC was used to determine the concentration of astragaloside Ⅳ. The effect of absorption site, drug concentration and the inhibitors of P-glycoproteon on the absorption had been studied. Result： By the testing of the statistics, the Ka and the Papp values of the duodenum, jejunum, ileum, colonic had significant differences （P 〈 0. 05 ）. The concentration from 20-80 mg ·L^-1 had no distinctive effect on the Ks and Papp of small intestine. The inhibitors of P-glycoproteon had no distinctive effect on the absorption of small intestine. Conclusion： Astragaloside Ⅳ is absorbed by typical passive diffusion mechanism.