大豆和大豆疫霉菌共培养体系的构建

Development of soybean and Phytophthora sojae co-culture system

本研究旨在建立一个适于分析大豆遭受大豆疫霉菌侵染后基因表达研究的互作体系。在比较了15个携带不同抗病基因的大豆基因型的组织培养情况和7个大豆疫霉菌分离物及其游动孢子单孢系的毒力变化的基础上,构建了大豆悬浮细胞和大豆疫霉菌游动孢子的共培养体系,进而分析了共培养过程中大豆细胞的活力和防御基因表达情况。结果表明,不同亲和力菌株的游动孢子引起的大豆细胞活力变化十分相似;非亲和菌株的游动孢子可诱导寄主防御基因的表达发生变化。此系统为深入开展大豆抗性机制研究提供了良好的平台。

The objective of the study was to develop an interaction system of soybean （Glycine max） and Phytophthora sojae in order to dissect the molecular events involved in response of the host plant to P. sojae contact and attack. Calli of 15 genotypes with differential resistance （Rps） genes were induced and sub-cultured. The virulence of 7 P. sojae isolates and their single-zoospore progenies on different soybean resistance genotypes were also tested. Finally, a soybean and P. sojae co-culture system was developed in vitro. The vigor and the defense gene expression of soybean cells at different time point after inoculation with P. sojae were analyzed. The results showed that the dynamic states of soybean cells inoculated with zoospores of compatible or incompatible P. sojae isolates were similar. Changes of expression of defense genes in soybean cells were also found in host and incompatible isolate co-culture system. The co-culture system is a good model to dissect the complex host defense response.