骨髓间充质干细胞共培养减轻顺铂所致的肾小管上皮细胞损伤

Protective effects of bone marrow mesenchymal stem cells against the renal tubular epithelial cell damage induced by cisplatin

目的:观察骨髓间充质干细胞对顺铂所致的肾小管上皮细胞损伤影响并探讨其机制。方法:①从小鼠骨髓中分离及鉴定间充质干细胞;②流式细胞仪检测顺铂所致肾小管上小管上皮细胞的坏死及凋亡状况。③骨髓间充质干细胞与肾小管上皮细胞共培养后,观察肾小管上皮细胞的坏死及凋亡状况。结果:细胞凋亡是顺铂致肾小管上皮细胞损伤的重要机制;骨髓间充质干细胞与肾小管上皮细胞间接共培养条件下,肾小管上皮细胞数量明显增高而肾小管上皮细胞的凋亡百分率明显降低;直接共培养较间接共培养肾小管上皮细胞数量增加更为明显。结论:骨髓间充质干细胞抑制顺铂诱导的肾小管上皮细胞凋亡及促进肾小管上皮细胞增殖,这一作用可能与骨髓间充质干细胞的旁分泌作用密切相关。

AIM： To investigate the protective effects and mechanism of mesenchymal stem cells on renal tubule cells treated by cisplatin. METHODS： The mesenchymal stem cells（MSCs） were separated from mouse bone marrow. The necrosis and apoptosis rate of renal tubule cells induced by cisplatin were detected by flow cytometry. After co - culture with MSCs, the above changes of renal tubule cells were detected. RESULTS ： In co - culture system, the apoptotic rates of renal tubule cell were decreased obviously and the cell number increased obviously. CONCLUSION： The MSCs protect the renal tubule cells from apoptosis induced by cisplatin. This effect may be related with the paracrine effects of MSCs.