中国狂犬病毒RdRp基因特点

Characterization of RdRp gene of rabies virus in China

目的 测定中国狂犬病毒人用疫苗株aG和减毒株CTN181的RdRp编码（L）基因序列并初步研究其结构和功能特点。方法 RT-PCR法获得若干交叉重叠小片段，序列拼接得到完整RdRp编码基因序列，利用生物学软件进行同源性和种系发生分析，并对RdRp保守功能序列和位点进行预测分析。结果 中国狂犬病毒ac和CTNl81株的L蛋白分别由6387和6384个核苷酸编码的2128和2127个氨基酸组成，前者比后者在起始部位多编码一个Met。结构功能分析发现在L蛋白上存在若干可能对RNA合成、mRNA聚腺苷酸化和磷蛋白磷酸化以及甲基转移酶的功能起决定作用序列；种系发生树显示中国疫苗株ac相对独立于WHO推荐使用的其他疫苗株。结论 完整L基因结构揭示，对于全面了解中国狂犬病毒分子特征，发现新的功能位点，开发以RdRp为靶标的新型抗病毒药物研究以及弹状病毒科种系发生分析提供了有力的工具。

Objective Characterization of RdRp gene （L） of rabies virus aG and CTNI81 strain in China. Methods Overlapped fragments were amplified and assembled. Then characterization and phylogenetic analyses as well as prediction of functional regions were performed using biologic softwares. Results L gene of CTNI81 and aG strains were composed with 6387nt and 6384nt respectively and there were two repeated ATG in the start of L gene in CTNI81 strain. In addition, some mutations and new functional regions were discovered and presumed to be crucial to the role of these regions in replication of rabies virus. Analyses of L gene provide new insight into the functional regions, and phylogenetic analyses showed that human vaccine strain aG were independent to others recommended by WHO. Conclusions The characterization of complete L genes will be heloful for research on new functional regions, antiviral drugs targeted at RdRo and phylogenetic analyses.