高致病性禽流感病毒H5N1安徽株A／Anhui／1／2005感染人肺癌上皮细胞（A549）的差异表达分析

The differential expression of the human lung carcinoma cells infected with high pathogenic avian influenza virus A/Anhui/1/2005 （H5NI）

目的 筛选人细胞中与高致病性禽流感病毒致病相关的基因，探讨高致病性禽流感病毒的致病机理。方法 分别用高致病性禽流感病毒安徽株和普通人流感病毒H1N1株分别感染人肺癌上皮细胞，通过人类全基因表达谱芯片技术对不同时段感染细胞进行差异表达分析，筛选出与高致病性禽流感病毒感染相关的候选基因，以实时定量荧光PCR法验证差异表达基因。结果 获得了不同致病性流感病毒感染细胞后的差异表达谱，验证了细胞凋亡通路、mTOR通路中以及与免疫相关的16个基因的差异表达。结论 H5N1感染后与普通人流感病毒H1N1相比具有促进细胞凋亡的趋势。

Objective To identify genes in human cells infected with high pathogenic avian influenza viruses H5N1. Methods The lung carcinoma cells line A549 was infected with H5N1 and H1N1, respectively. We harvested the infected cells at the different time points after infection and screened the genes with differential expression via microarray technology. The candidate genes were selected and confirmed by quantitative real-time PCR. Results The spectrum of genes with the differential expression in the cells infected with H5N1 was obtained and 16 candidate genes were identified in the cellular apoptosis pathway, mTOR pathway, and the cellular immunity as well. Conclusions Our results suggest that H5N1 exert a stronger impact on eliciting apoptosis of infected cells than the common influenza virus H1N1.