CGRP和NGF对全脑缺血再灌注大鼠脑组织PKB表达的调节作用

Effect of CGRP and NGF on the Expression of PKB during Whole Cerebral Ischemia and Reperfusion in Rats

目的:研究大鼠脑缺血再灌注后蛋白激酶B(protein kinaseB,PKB)的表达,探讨降钙素基因相关肽(CGRP)和神经生长因子(NGF)对脑组织缺血再灌注的保护作用及机制。方法:根据Nagasawa线栓法制作大鼠右侧大脑中动脉栓塞(MCAO)模型,采用电镜、免疫组织化学SABC法及显微图像分析检测海马及皮质内PKB的表达。结果:大鼠缺血再灌注海马及顶叶皮质内PKB反应产物较正常组增多(P<0.05),而注射CGRP或NGF后阳性产物明显高于缺血再灌注组(P<0.01),二者联合应用效果更加显著(P<0.05)。结论:CGRP及NGF参与缺血神经元PKB的调节,二者对缺血神经元有协同修复作用。

Objective： To investigate the expression of protein kinase B （PKB） in hippocampus after regional cerebral ischemia and reperfusion in rats, explore the protective effects and mechanism of calcitonin and gene-related peptide （CGRP） and nerve growth factor （NGF） on brain tissue. Methods.. An novel model of regional cerebral ischemia and reperfusion induced by middle cerebral artery occluded （MCAO） in rats was mode. The expression of PKB in hippocampus and cortex was detected with electron microscope, SABC immunohistochemical method and analyzed by microimage system. Results： The expression of PKB in hippocampus and cortex was increased after cerebral ischemia and reperfusion （ P 〈 0.05）. Compared with this, the percentage of PKB immunoreaction positive cells in CGRP or NGF group were higher （P 〈 0.01）. The combined usage of CGRP and NGF enhanced such effects （P 〈 0.05）. Conclusion： The results indicate that CGRP and NGF participate in the regulation of expression of PKB in ischemic neurons, and they may cooperate with each other.