成年期甲状腺功能减退对雄性小鼠海马神经颗粒素表达的影响

Effect of adult-onset hypothyroidism on neurogranin expression in hippocampus of adult male mice

成年雄性昆明种小鼠分为对照组、丙基硫氧嘧啶(PTU)组、PTU+T_4组,造模8周后,免疫组化和Western印迹法检测神经颗粒素(Ng)表达,发现甲状腺功能减退(甲减)小鼠海马区Ng表达降低,用T_4治疗可使之恢复,提示海马内Ng减少可能是成年期甲减雄性小鼠认知功能受损的机制之一。

Adult male Kunming mice were divided into normal control group, propylthiouracil （PTU） treated group and PTU ＋ T4 treated group. Neurogranin （Ng） protein expression in the hippocampus after 8 weeks was assayed by Western blotting and immunohistochemical method. Results showed that Ng protein was decreased in the hippocampus of male mice with adult-onset hypothyroidism, suggesting that decreased Ng, which is correctable by T4, is involved in cognitive dysfunction in adult-onset hypothyroid male mice.