1-脱氧野尻霉素对高糖刺激大鼠系膜细胞增殖的影响

Effect of 1-deoxynojirimycin on high glucose-stimulating proliferation of rat mesangial cells

观察1-脱氧野尻霉素(1-deoxynojirimycin,DNJ)对大鼠系膜细胞增殖的影响及进行机制探讨。结果显示,DNJ抑制高糖刺激的系膜细胞增殖呈剂量和时间依赖性。DNJ可降低高糖刺激的系膜细胞α-平滑肌肌动蛋白(α-SMA)、整合素β1 mRNA、蛋白及黏着斑激酶(FAK)的蛋白表达(P<0.05或P<0.01),抗整合素β1抗体、FAK抑制剂genistein也可抑制高糖刺激系膜细胞α-SMA的蛋白表达(均P<0.01)。

The effect of 1-deoxynojirimycin （DNJ） on the proliferation of rat mesangial cells was observed and its mechanism was explored. The results showed that DNJ significantly inhibited the proliferation of rat mesangial cells induced by high glucose in time- and dose-dependent manners. DNJ significantly decreased expressions of a-smooth muscle action （ α-SMA ）, integrin β1 mRNA and protein and focal adhesion kinase （FAK） protein stimulated by high glucose in rat mesangial cells （P 〈 0.05 or P 〈0. 01 ）. Anti-integrin β1 antibody and genislein markedly reduced the expression of α-SMA protein stimulated by high glucose as well （ all P 〈0.01）.