玉米麦根酸测定方法研究

Study on the Methods for the Determination of Corn Root-Washing's Mugineic Acid

禾本科作物麦根酸类物质(MAs)分泌量的准确、快速测定,对于铁营养高效育种及缺铁耐性的生理研究具有重要价值。以玉米自交系为材料,根据麦根酸类物质的溶铁特性,建立了ICP-AES间接测定麦根酸类物质的方法,改良了脱氧麦根酸(DMA)的UV-HPLC直接测定法,并与传统的比色法测定结果进行比较。结果表明,采用ICP-AES法,以Fe2O3粉末作为活化对象,与采用Fe(OH)3的传统方法测定结果相比,结果稳定,t(ICP-AES)=4.080>t0.05(15)=2.131重复性好,RSD(n=3,%)=3.3,说明ICP-AES法是评价玉米及其它禾谷类作物麦根酸类物质分泌量的一种简单、快速的方法。改良的UV-HPLC法也具有准确、高效的特点,可应用于禾谷类作物DMA分泌量的直接测定。

The accurate and quick measurement of mugineic acids （MAs） of graminaceous plants was very important for the breeding of high-efficient usage of iron nutrition and physiological research of Fe-deficient tolerance. According to the chelate characteristic of Fe（ Ⅲ ） with mugineic acid, a new indirect method of inductively coupled plasma-atomic emission spectrometry （ICP-AES） and a modified direct method of UV detection-high performance liquid chromatography （UV-HPLC） for the measurement of MAs or deoxymugineic acid （DMA） were established in corn inbred lines. The comparison of these two methods with traditional spectrophotometry was also made. The results showed that the ICP-AES method, using Fe2O3 as the dissoluble object instead of the traditional Fe （OH） 3, was more stable t （ICP-AES） = 4. 080 〉 t0.05 （ 15 ） = 2. 131 and repeatalble （RSD（n = 3） = 3.3）. It was suggested that ICP-AES was a simple and quick method for evaluation of the MAs content in corn and other graminaceous crops. The modified UV-HPLC was also precise and efficient, and could be used for the direct mesurement of graminaceous＇s deoxymugineic acid.