鲍曼不动杆菌老年患者分离株中发现16SrRNA甲基化酶基因新亚型

A new subtype of 16S rRNA methylase gene by Acinetobacter baumannii isolated from the elderly patients

目的了解鲍曼不动杆菌老年患者分离株16S rRNA甲基化酶和氨基糖苷类修饰酶基因型。方法采用PCR检测20株鲍曼不动杆菌老年患者分离株12种16S rRNA甲基化酶和氨基糖苷类修饰酶基因。结果7株检出16S rRNA甲基化酶基因(armA),19株检出氨基糖苷类修饰酶基因[其中aac(3)-I阳性率为95%,ant(3")-I为95%,aac(3)-II为40%,aac(6′)-Ib为15%)]。6号株armA基因测得序列翻译成氨基酸序列与美国核酸库(GenBank)已登录的armA氨基酸不同,为新亚型。结论本组鲍曼不动杆菌老年患者分离株95%携带16S rRNA甲基化酶和氨基糖苷类修饰酶基因。

Objective To investigate the genotype of 16S rRNA methylase and aminoglycoside-modifying enzymes in Acinetobacter baumannii isolates in the elderly patients. Methods The genes of 12 kinds 16S rRNA methylase and aminoglycoside-modifying enzymes were detected by polymerase chain reaction （PCR）. Results In 20 strains, 7 strains carried 16S rRNA methylase gene （arrnA）; 19 strains were positive for the genes of aminoglycoside-modifying enzymes [the positive rates of aac （3）-I, ant （3^n）-I, aac （3）-II and aac （δ′）- Ib were 95%, 95%, 40% and 15% respectively]. Serial change appeared of amino acids in the sequence of armA of No. 6, which identified itself as new subtype. Conclusion 95% of the Acinetobacter baumannii iso- lates in the elderly patients were carrying 16S rRNA methylase and aminoglycoside-modifying enzymes.