临床型乳房炎与正常奶牛血浆的差异蛋白质组研究

Comparative Proteomics Analysis of Plasma Between Healthy Dairy Cows and Clinical Mastitic Cows

【目的】检测和分析临床型乳房炎与正常奶牛血浆中差异表达的蛋白质。【方法】用二维凝胶电泳分离血浆蛋白,凝胶用硝酸银染色和考马斯亮蓝染色,PDQuest7.4软件自动检测分析差异表达蛋白斑点并经高效液相色谱串联离子阱质谱鉴定。【结果】凝胶图谱分析后得到3个差异蛋白斑点,鉴定为2种蛋白质(结合珠蛋白前体和乳腺球蛋白)。结合珠蛋白前体在临床型乳房炎奶牛血浆中表达量增加,可作为乳房炎诊断标记分子;而乳腺球蛋白的表达量下调。【结论】临床型乳房炎与正常奶牛的血浆中蛋白质的表达存在差异,对差异表达蛋白质的研究有利于探索机体的生理病理状态,可为疾病的诊断和治疗提供全面的信息。

[Objective] Differential proteomic expressions in plasma from healthy dairy cows and clinical mastitic cows was investigated. [Method] The current research presents the protein changes using two-dimensional gel electrophoresis （2-DE） after stained with silver nitrate and colloidal coomassie blue, differential expression proteins were detected by PDQuest 7.4 software and subjected to ion trap mass spectrometer equipped with a Surveyor HPLC system, and differential spots of protein were identified. [Result] Three protein spots were identified to be two proteins differentially expressed. Overall, haptoglobin precursor was up-regulated in cows infected with clinical mastitis, could serve as a mastitis-associated diagnostic marker. Protein SCGB 2Al （secretoglobin, family 2A, member l） was down-regulated. [Conclusion] There was a significant difference at protein level in plasma from healthy dairy cows and clinical mastitic cows. Differential proteome expression analysis may be useful to clarify the mechanisms involved in pathophysiology, and to further study the related proteins and the molecular markers of clinical mastitis.