赤子爱胜蚓谷胱甘肽和丙二醛含量变化指示重金属污染土壤的生态毒性

VARIATION OF GSH AND MDA LEVELS IN EISENIA FETIDA AS INDICATOR OF ECO-TOXICITY OF HEAVY METAL CONTAMINATED SOIL

为探讨赤子爱胜蚓谷胱甘肽(GSH)和丙二醛(MDA)含量变化对重金属污染土壤生态毒性的指示作用,在试验室模拟条件下,利用人工土壤和自然土壤,研究了重金属含量与蚯蚓GSH和MDA含量变化的剂量效应关系。结果显示,在人工土壤试验中,Cu可诱导蚯蚓GSH和MDA含量上升,暴露2 d、7 d、14 d后,Cu浓度与蚯蚓GSH含量的相关系数分别为0.556、0.807和0.775,与MDA含量的相关系数分别为0.760、0.672和0.544,均存在显著的剂量效应关系(p<0.05)。自然土壤试验结果显示,赤子爱胜蚓的GSH和MDA含量与土壤Cu全量的偏相关系数分别为-0.830(p<0.001)和-0.599(p<0.05),与Cd全量的偏相关系数分别为-0.697(p<0.05)和-0.690(p<0.05)。同时发现,赤子爱胜蚓GSH含量变化对土壤重金属响应的敏感性要高于MDA。

In order to explore potential of the variation of GSH and MDA levels in Eiseniafetida as bio-indicators in monitoring heavy metal contamination of soil, effect of copper on Eiseniafetida was studied using artificial soil and contaminated agricultural soil. Results show that the levels of GSE and MDA in E. fetida are significantly related to copper in the artificial soil, with correlation coefficients being 0. 556, 0. 807 and 0. 775 between Cu concentration and levels of GSH and 0. 760, 0. 672 and 0. 544 between Cu concentration and levels of MDA, respectively after exposure of the worms for 2, 7 and 14 days. Both showed close close-effect relationship. In natural soil, partial relationship was found between Cu content and levels of GSH and MDA in the worms with correlation coefficients being - 0. 830 （p 〈 0. 001 ） and - 0. 599 （p 〈 0. 05 ） , respectively, and between Cd content and levels of GSH and MDA in the worms as well, with correlation coefficients being - 0. 697 （p 〈 0. 05 ） and - 0. 690 （ p 〈 0. 05） , respectively. Moreover, it was also found that GSH level in the worms was more sensitive than MDA level to soil heavy metals.