亚低温对雨蛙肽致大鼠胰腺腺泡细胞损伤的保护作用

Protective effect of mild hypothermia on caerulein-induced injury of pancreatic acinar cells

目的观察亚低温对雨蛙肽刺激大鼠胰腺腺泡细胞的保护作用,并探讨其机制。方法取大鼠胰腺组织块,分为常温对照组、亚低温对照组、常温雨蛙肽组和亚低温雨蛙肽组。分别测定不同时间点(15、30、60、90min)上清液中淀粉酶、脂肪酶和组织匀浆液中胰蛋白酶活性、乳酸脱氢酶(LDH)漏出率及胰腺腺泡细胞内钙离子([Ca^(2+)]i)浓度,并测定1、3、5 h的胰腺细胞存活率。结果与常温雨蛙肽组比较,30、60和90 min时亚低温雨蛙肽组的淀粉酶和脂肪酶均显著降低(P值分别<0.05、0.01),15和30 min时胰蛋白酶活性显著降低(P值分别<0.05、0.01),60和90 min时LDH漏出率显著降低(P值分别<0.05、0.01),30、60和90 min时胰腺腺泡细胞内[Ca^(2+)]i浓度显著降低(P值分别<0.05、0.01),3和5 h时胰腺细胞存活率显著升高(P值均<0.05)。结论亚低温对雨蛙肽刺激的胰腺腺泡细胞具有保护作用,其机制可能与抑制胰蛋白酶原激活、减轻细胞内钙超载有关。

Objective To investigate the protective effect of mild hypothermia on supramaximal caerulein- induced injury of pancreatic acinar cells and to explore the related mechanism. Methods Pancreatic samples of rats were divided into 4 groups; normothermia-control group, hypothermia control group, normothermia-caerulein group and hypothermia-caerulein group. The activities of amylase, lipase in the supernatant, the activity of trypsin in homogenate, the intracellular Ca^2＋ concentration, and lactate dehydrogenase （LDH） leakage were examined at different time points（15, 30, 60, and 90 min）; the cell survival was determined 1, 3, and 5 h after caerulein treatment. Results The activities of the amylase, lipase （30, 60, and 90 min）, trypsin （15, 30 min）, the LDH leakages （60, 90 min） were significantly lower, the cell survival （3, 5 h） was higher in the hypothermia-caerulein group than in the normothermia-caerulein group （P〈0.05 or 0.01）. Conclusion Mild hypothermia can protect pancreatic acinar cells from caerulein-induced injury; the mechanism might be related to the inhibition of trypsinogen activation and mitigation of calcium overloading.