小球藻与2株藻际异养细菌相互作用的研究

A Study on the Interaction Between Chlorella Vulgaris and Heterotrophic Bacteria in Phycosphere

探讨了2株海洋细菌Z-QD08、Z-QS01与小球藻的相互作用关系.Z-QS01抑制小球藻的生长,低密度Z-QD08促进小球藻的生长,达到107cells/mL时小球藻受到抑制,Z-QS01优势组对生长初期的小球藻有抑制作用.Z-QD08优势组和等量混合组则促进小球藻的生长,生长后期均促进小球藻的生长且Z-QD08占优势.对数期的小球藻抑制Z-QS01的生长(p<0.05),对Z-QD08有促进作用;混合培养体系中小球藻选择性抑制Z-QS01.实验结果可为生态调控防病技术在海水养殖中的应用以及发展微生态制剂提供理论支持.

Interaction between chlorella vulgaris and two strains of marine heterotrophic bacteria （Z-QD08 and Z-QS01） was studied. Z-QS01 showed inhibitory effect on C vulgaris and the inhibitory effect was enhanced gradually with increasing density of Z-QS01. Z-QD08 stimulated the growth of C vulgaris at low density, and presented an inhibitory effect when the density of Z- QD08 reached 10^7cells/mL. There were obvious different effects on C vulgaris among different compounding ratio groups of Z QD08 and Z-QS01 in the initial phase. The dominant group of Z- QS01 restrained the growth of C vulgaris, while the dominant group of Z-QD08 and equivalent group showed stimulative effects to C vulgaris. Yet all groups showed stimulative effects to C vulgaris in the terminal phase. C vulgaris decreased the cell density of Z-QS01 while stimulated the growth of Z-QD08 in the exponential, growth phase. C vulgaris demonstrated selective inhibitory effect to Z-QS01 in the mixed culture system. Experimental results offered theoretical support to apply ecological control in marine culture and to develop microbial preparation.