温度对芥蓝幼苗叶片蛋白质和核酸的含量的影响

Effect of temperature on soluble protein and nucleic acid of leaves in Chinese Kale(Brassica alboglabra Bailey)

以中花芥蓝为材料,设置T1(18/12℃),T2(24/18℃),T3(30/24℃),T4(36/30℃)4个不同温度处理.研究了温度对芥蓝蛋白质和核酸含量的影响.结果表明,可溶性蛋白质含量以T4处理最高,T1处理次之,T3处理再次,T2处理最低.随着温度处理时间的延长各处理样品的可溶性蛋白质含量出现先上升后下降的消长变化.茎端核酸,DNA,RNA含量随着温度升高而下降.随着温度处理时间的延长,各处理样品的核酸、RNA含量除T4处理持续下降外,其他三个处理下降后有所回升;而DNA含量随着处理时间的延长,除T1处理下降后上升外,其他三个处理表现为降—升—降的变化趋势.低温可提高核酸的合成,维持核酸的稳定性,高温不利于核酸,DNA,RNA的合成,导致核酸含量的下降.

The effects of four different temperature such as T1 （18℃ day/12℃ night）, T2 （24℃ day/18℃ night）,T3（30℃ day/24℃ night）,T4（36℃ day/30℃ night） treatments on soluble protein and nucleic acid were studied in Chinese Kale. The results showed that the content of soluble protein was the highest in 36℃ day/30℃ night treatment and the lowest in 30℃ day/24℃ night treatment. The contents of total nucleic acid,DNA and RNA in the stem apex decreased with prolongation of temperature, with prolongation of temperature treatment was prolonged,the contents of total nucleic acid and RNA in all treatments rose gradually after decreasing except for 36℃ day/30℃ night treatment which decreased continuously. The content of DNA showed a changing trend such as decreasing-increasingdecreasing except for 36℃ day/30℃ treatment which increased after decreasing. Low temperature could accelerated the synthesis of nucleic acid, maintained the stability of the nucleic acid,but high temperature inhibited the synthesis of the nucleic acid,DNA and RNA, and subsequently led to the decreasing of nucleic acid contents.