高原低氧环境下大鼠血浆抗利尿激素水平变化研究

Effect of high altitude hypoxia on plasma vasopressin in rats

目的:研究高原低氧环境下大鼠血浆抗利尿激素(PAVP)水平变化,探讨其与急性高原病的关系。方法:将50只雄性SD大鼠随机分为5组,分别为24 h4、8 h7、2 h1、w及1个月(A、B、C、D、E)组,同时设立对照组(西宁地区,海拔2260m),将5个实验组由西宁地区带至青海大学可可西里高原医学研究基地(海拔4600 m),在不同时间点处死取材,应用放射免疫法检测PAVP的浓度,并与对照组比较。结果:PAVP浓度在初入高原后呈下降趋势,在48 h达到最低,后逐渐回升,至1 w时已经接近对照组水平,1个月时达到最高水平。结论:高原慢性低氧环境下,PAVP的浓度随着缺氧时间的延长而变化,此可能与机体缺氧程度及高原低氧习服过程有关。

Objective： To investigate the changes of plasma vasopressin （PVAP） in rats exposed to hypoxia at high altitude, Methods： 50 male SD rats were randomly divided into five testing groups and one control group, The testing groups were transported to Ke - ke - xi - li Natural Reservation areas （4600 m） in Qinghai Province. Five testing groups were sacrificed and their tissue samples were gained after they lived at high altitude for 24 h, 48 h, 72 h, 7 d and I month（A, B, C, D, E group）, respectively, The control group was treated in Xining city （above sea level 2260 m）, The concentration of PVAP of both testing groups and control group were measured by radio - immunity method, Results：The concentration of PVAP in five testing groups were 142. 46±10.57, 86. 94±6. 49, 169, 65±11.15, 306. 46±11. 14 and 360, 29±11.20 respectively,it reached the lowest level at 48 h, then rose gradually,reached the highest level at 1 month （P〈0.05） and was similar to that of the control group at 7 d. Conclusion： The PVAP level will alter after the rats are exposed to high altitude,it decreases in early period and increases in later period, This change seems to be related to degree of hypoxia and adaption to high altitude in rats.