摘要
利用琼脂糖(agarose)将过氧化氢酶(Cat)固定在热裂解石墨电极表面,制备Cat-agarose膜修饰电极.包埋在琼脂糖中的过氧化氢酶与电极直接传递电子.在pH6.0的缓冲溶液中可得一对可逆的氧化还原峰,式电势为-0.343V(vs SCE),这是过氧化氢酶辅基血红素Fe(III)/Fe(II)电对的氧化还原.其式电势随溶液的pH值增加而负移且成线性关系,直线斜率为-36.8mV/pH,说明过氧化氢酶的电子传递过程伴随有质子的转移.过氧化氢酶修饰电极.在16.5-278μmol/L的范围内,催化电流与过氧化氢浓度成线性关系.
Cat-agarose film modified edge-plane pyrolytic graphite electrode （EPG） was made by entrapping catalase （Cat） in agarose hydrogel at the EPG surface. Cat confined in agarose film can directly transfer electron with EPG. A pair of redox peaks was observed in pH6.0 buffer. The formal potential is -0. 343V, corresponding to CatFe（Ⅲ） /Fe （Ⅱ） redox couple. The formal potential of Cat Fe（Ⅲ） /Fe（Ⅱ） couples in agarose membrane shifted linearly with a decrease of pH. The value of slope of is --36.8mV/pH, which suggests that one proton transfer in the one--electron reaction. The Cat--agarose modified EPG exhibits the electrocatalytic activity to oxygen and hydrogen peroxide. The cathodic peak current increase linearly with the concentration of H2O2 in the range of 16.5--278μmol/L.
出处
《郧阳师范高等专科学校学报》
2008年第3期36-38,共3页
Journal of Yunyang Teachers College
关键词
过氧化氢酶
电催化
直接电化学
双氧水
Catalase
Electrocatalysis
Direct electrochemistry
Hydrogen peroxide
