中草药光敏剂的筛选及对人胃癌细胞系光动力学杀伤效应的研究

Screening New Photosensitizers from Chinese Medicinal Herbs and Searching for Herbal Photodynamic Killing Effects on Human Stomach Cancer Cells

目的：筛选中草药抗癌光敏剂，寻求中药用于肿瘤光动力学疗法新途径的可能性。方法：检测了１３种中草药提取液的荧光激发波长、发射波长；它们被ＢＧＣ－８２３人胃癌细胞（简称８２３细胞）摄取后的细胞荧光强度；提取液在固定细胞和活细胞染色中荧光物质的分布。亲和部位，以及ｐＨ值对提取液染色细胞荧光强度的影响。在此基础上，进一步选择性进行了光敏抗癌实验。结果：１３种中草药中，黄柏、黄连为最理想的荧光剂。与加药不照光的对照组比较，黄连在照光实验组，使８２３细胞代谢活力、增殖能力明显降低，并使癌细胞死亡率明显增高。结论：中药具有成为新的抗癌光敏剂的潜力和将来用于肿瘤光动力学疗法的可能性。

Objective: To find new photosensitizers from Chinese medicinal herbs for cancer photodynamic therapy. Methods: The extracts of thirteen herbs were examined: (1 )Their fluorescence excitation wave lengths and emission wave lengths; (2 )Their fluorescence intensity in living cells and (3 ) Their distribution and localization in the living cells and the fixed cells both stained in each extract, and responses of cell fluorescence intensity to pH value change.Furthermore, the herb's anticancer photosensitive efficiencies were studied by using BCG-823 human stomach cancer cells. Results: Cortex phelledendri and Rhizoma Coptidis, were found with optimal fluorescence properties as photosensitizers in this test. The latter could remarkably reduce the cell metabolic viability, proliferative ability and increased the cell mortality when the cells exposed to both drugs and luminance but not only to drug. Conclusions:The potential of Chinese medicine as new kind of photosensitizer and its possibility for use in anticancer photodynamic therapy are existed.