以18S rDNA序列鉴定一株产纤维素酶真菌

Identification of a Cellulose-producing Fungi based on 18S rDNA Sequence

采用18S rDNA序列分析和形态鉴定对一株具有较高纤维素酶活性的真菌HY12进行了鉴定。18S rDNA序列分析表明,菌株HY15与木霉属多株菌的18S rDNA的同源性均在99.0%以上,而与其中的绿色木霉(Trichoderma viride)同源性达到100.0%。从构建的系统进化树中可以看出,HY12与Trichoderma viride共同构成一个分支,并与哈茨木霉(Trichoderma harzianum)聚成一大枝。该菌形态鉴定与绿色木霉比较接近,因此初步确定菌株HY12为绿色木霉菌。

A fungi HY12, possessing high cellulose activity, was identified by 18S rDNA sequence and morphological observation. The sequence data was compared with the published sequences of relative fungi strains in Genbank. The results showed that the homology of the 18S rDNA sequences of strain HY12 with those of several Thichoderma strains was more than 99.0%. As shown in the phylogenetic tree, strain HY12 and Trichoderma viride constituted a branch and they constituted a high-order branch together with Trichoderma harzianum. Besides, the strain HY12 was similar with Trichoderma viride in morphological character. So the strain HY 12 belonged to the species of Trichoderma viride.