摘要
本文建立了应用PCR技术鉴别腊肠中植物成分的方法。通过前处理与酚仿抽提DNA的方法相结合,建立了一种快速简便的从腊肠中提取DNA的方法,整个DNA提取的过程时间为2h左右,提取的DNA浓度和A260/A280均达到了PCR的要求。通过PCR分析,发现从腊肠中提取DNA完全可以进行PCR检测,并且该方法可定性检测腊肠中的植物成分。
In this article, a rapid and simple method for DNA extraction from sausage was developed and. PCR was used in detection of plant element in sausage. The time of DNA extraction via phenol-chloroform extraction of the pretreated sausage was 2 h and the quality and concentration of the achieved DNA met the demand of PCR. The plant element in sausage could be detected qualitatively by this method.
出处
《现代食品科技》
EI
CAS
2008年第7期722-724,共3页
Modern Food Science and Technology
基金
广东省科技计划项目(No2007A020300007-5、2007B080501009、2007B080401015)
