应用化学抑制剂和酶偶联比色法直接测定LD_1

Direct measurement of LD_1 by enzyme linked photometry with chemical inhibitor

本文应用1－6已二醇作为LD分子中M亚基的专一抑制剂，采用双试剂法，由自动生化分析仪直接将一定浓度的1．6－己二醇溶液（第一试剂）与血清样品同时加入反应比鱼池（加人1．6已二醇的最终浓度为1．16mol／L），于30C保温5分钟后再加入第二试剂，用酶偶联比色法测定LD；。LD同工酶电泳图谱表明在上述条件下，LD2～LDs的活性全部被抑制。用本法同常规电泳法平行检测51例血清LD，结果相关良好．r＝0．9885，Y＝0．87X＋189；平均回收率104.4％，线性范围至少可达到470U／L；批内CV（％）为3．83～4．08。

1, 6-Hexanediol was used as a specific inhibitor for 1actate dehydrogenase (LD) M subunit in the mea-surement of LD1 isoenzyme. The first reagent containing certain concentration of 1, 6-hexanediol and serumsample were added into the reaction cup at same time on autoana1yzer(the fina1 concentration of 1, 6-Hexane-diol was 1. 16 mo1/L) and incubated at 30℃ for 5 min before adding the second reagent,and finaI1y the LD1activity was measured by enzyme linked photometry. The electrophoresis pattern of LD isoenzymes showedthat LD2-LDs activities were totalIy inhibited (under above condition). Good correlation was achieved in thecomparison between our method and the routine electrophoresis method for 5l sera (r= 0. 9885,Y= 0. 87X+18. 9). The average recovery was lO4. 4%,the linear range was up to 47O U/L at least and the CV(%)ofwithin run was 3. 83-4. 08.