反相高效液相色谱法同时测定洁尔阴、妇炎栓中苦参碱和氧化苦参碱

Simultaneous Determination of Matrine and Oxymatrine in Jieeryin and Fuyanshuan by RPHPLC

用反相高效液相色谱法分离并测定洁尔阴、妇炎栓中的苦参碱和氧化苦参碱，建立制剂中苦参碱、氧化苦参碱分离、测定的色谱方法。色谱条件：ＯＤＳ柱，流动相为甲醇水三乙胺（５５∶４５∶００２），紫外检测波长为２１５ｎｍ。方法简便、灵敏、准确，为制剂的质量评价、质量控制提供了依据。

Matrine and oxymatrine in Jieeryin and Fuyanshuan were separated and determined by Reversed Phase High Performance Liquid Chromatography,A chromatographic method for the separation and determination of matrine and oxymatrine in these preparations was established.Chromatographic coditions:ODS column with methanolwatertriethylamine(55∶45∶0.02) as the mobil phase,and UV detection at 215nm.The method is simple,sensitive and accurate.This study provides the scientific basis for quality evaluation and quality control in making these preparations.