单结合胆红素水溶解度及其与胆石形成关系的探讨

The water solubility of monoconjugated bilirubin and its possible role in the formation of gallstones

目的：从单结合胆红素（monoconjugatedbilirubin，MCB）水溶解度方面探讨双结合胆红素（diconjugatedbilirubin，DCB）与胆石形成的关系．方法：在制备出大量较纯的MCB的基础上，首次测定了DCB、MCB和非结合胆红素（unconjugatedbilirubin，UCB）等在37℃，pH7．9条件下的水溶解度．结果：DCB、MCB和UCB的水溶解度分别为3410．90±2．42μmol／L、558．25±5．96μmol／L和12.43±0.32μmol／L；MCB水溶解度仅为DCB的1／6．结论：MCB在胆汁沉淀和结石形成中可能起着重要的作用．

Objectives:To discuss the relationship between monoconjugated bilirubin(MCB) andformation of gallstones. Methods:With preparing a larger amount and a rather high purity ofdiconjugated bilirubin(DCB) and MCB, the water solubilities of DCB, MCB and unconjugatedbilirubin(UCB) were measured by HPLC. Results:The water solubilits of DCB, MCB and UCB were3410.90±2.42μmol/L, 558.25±5.96μmol/L and 12.43±0.32μmol/L, respectively, at 37℃ pH7.9. Theaqueous MCB solubility at 37℃ pH7.9 is about six-folds lower than that of DCB. Conclusions:thatMCB, the main pigment in the lithogenic bile, may exert a critical role in the formation ofgallstones.