溃疡性结肠炎患者肠神经内分泌细胞的观察

Neuroendocrine cells study in patients with ulcerative colitis

目的：观察溃疡性结肠炎患者肠切除标本中神经内分泌细胞的变化。方法：应用免疫组织化学和形态计量学方法。结果：病变肠组织VIP能神经，S-100蛋白及神经特异性烯醇化酶免疫阳性神经元、神经纤维形态异常，其线密度（Lv％）、体密度（Vv％）增高（分别P＜0．01）。生长抑素、血清素免疫阳性细胞及肠嗜银细胞数量明显减少（分别P＜0．01）。结论：肠神经内分泌细胞的异常可能参与溃疡性结肠炎的发生和发展。

Objective:To observe the changes of neuroendocrine cells in patients withulcerative colitis. Methods:Use immunohistochemical staining for vasoactive intestinal peptide(VIP), S-100 protein, neuronal specific enolase (NSE), endocrine cells of somatostatin andserotonin, also histomorphometric analysis in surgical specimens taken from 16 cases of ulcerativecolitis (UC) and 10 controls. Results:As compared with controls, the mucosal nerve fiberscontaining VIP and NSE were thickened, the linear densities (Lv%) were increased (P<0.01).Furthermore, in both submucosa and myenteric plexus, nerve fibers and neurons containing VIP, S-100 protein and NSE were hyperplastic and thickened, the volumetric densities (Vv%) were alsoincreased (P<0.01). the number of positive immun-osomatostatin serotonin cells and argyrophilcells were diminished in the affected mucosa. (P<0.01) Conclusions:It suggests that entericneuroendocrine cells abnormalities might play an important role in the pathogenesis of UC.