铁、牛磺酸和精氨酸对大鼠肝纤维化的影响

Effect of iron,taurine and arginine on rat hepatic fibrosis

应用放射性大鼠肝纤维化模型探讨铁剂在放射性肝纤维化发生中的作用及牛磺酸和精氨酸对肝脏的保护作用。方法将大鼠分为０Ｇｙ（正常对照）、３０Ｇｙ和３０Ｇｙ＋铁剂、３０Ｇｙ＋牛磺酸和３０Ｇｙ＋精氨酸组。于肝区照射后１，３个月分别检测血清铁离子、肝组织羟脯氨酸（Ｈｙｐ）和丙二醛（ＭＤＡ）含量以及应用偏振光显微镜观察肝内Ⅰ、Ⅲ胶原纤维的生成及分布特点。结果铁剂能明显增加肝组织中ＭＤＡ的含量和血清铁离子浓度。照射后１个月，铁剂组肝组织中Ｈｙｐ开始明显增加，进而肝脏中胶原纤维产生增多。牛磺酸和精氨酸能明显降低血清铁离子浓度，减少肝组织Ｈｙｐ含量。结论外源性铁剂能促进放射性肝纤维化的早期发生；

Objective\ The promotive role of iron on pathogenesis of hepatic fibrosis and the protective role of taurine and L arginine against hepatic fibrosis were studied.Methods\ The model of rat radiation hepatic fibrosis was used.Experimental rats were divided into 0Gy,30Gy,30Gy+iron,30 Gy+taurine and 30Gy+L arginine groups.Serum iron,liver tissue hydroxyproline(Hyp) and malondialdehyde (MDA) were measured one and three months respectively after irradition of hepatic tissue,production and distribution characteristics of hepatic tissne type Ⅰ and Ⅲ collagen were observed with a polarizing microscope.Results\ Administration of iron agent could significantly increase hepatic tissue MDA content and serum iron concentration,one month after irradiation ,hepatic tissue Hyp in 30Gy+iron group began to increase,and collagen in hepatic tissue obviously increased.Taurine and L arginine could reduce serum iron concentration and decrease production of hepatic fissue Hyp.Conclusion\ Exogenous iron agent could promote early development of radiation hepatic fibrosis;taurine and arginine could diminish pathologic alteration of hepatic fibrosis to a certain extent.