通络方剂对1型糖尿病大鼠模型肾脏保护作用的形态学研究

Morphologic study on the protection function of Tongluo decoction on kidney in rat models of type 1 diabetes mellitus

目的观察通络方剂对链脲佐菌素(STZ)诱导的1型糖尿病大鼠肾脏病理改变的影响。方法SD大鼠给予STZ以50 mg/kg腹腔注射建立1型糖尿病大鼠模型,随机分成糖尿病组、通络方剂治疗组。另选9只大鼠作为正常对照组。通络方剂治疗组给予通络方剂1.0 g/(kg.d)灌胃,其余大鼠予以等量蒸馏水灌胃,3组大鼠在相同环境条件下饲养。6周后处死,取肾脏行HE染色以及透射电镜观察肾皮质组织病理改变。结果糖尿病组大鼠与正常对照组大鼠相比,光镜及电镜下已出现糖尿病肾病病理改变,而通络方剂组肾皮质病理病变较轻。结论通络方剂对STZ诱导的1型糖尿病大鼠肾脏具有一定的保护作用。

Objective It is to observe the effect of Tongluo decoction on pathologic changes of kidney in rats with diabetes mellitus （DM） induced by streptozotocin （STZ）. Methods SD rats were given STZ 50 mg/kg by intraperitoneal injection to make models of 1 tpye DM. Then the models were randomly divided into DM group and Tongluo group. Another 9 rats were chosen as normal control group. Rats in Tongluo group were given Tongluo decoction 1.0 g/（kg·d ） by intragastric administration. The rats in other groups were given distilled water of the same dosage by intragastric administration. All the rats in the three groups were fed under the same conditions for 6 weeks, then they were killed to get kidney to do HE staining and to observe pathologic changes under electron microscope. Results Compared with normal control group, there were pathologic changes under light microscope and electronic microscope in DM group, but the changes in Tongluo decoction group were slight. Conclusion Tongluo decoction has a preventing function on kidney in STZ-induced diabetic rats.