三种草坪草愈伤组织的诱导及其脱水处理后的蛋白分析

Callus induction of three turf grass and analysis of their proteins after dehydration treatment

研究了匍匐翦股颖、高羊茅和狗牙根3种草坪草愈伤组织诱导情况,愈伤组织诱导率分别为53.0±8.7%、60.7±7.9%和14.7±4.0%,三者的愈伤组织含水量约为91.4±0.3%、94.1±0.4%和93.4±0.2%.经空气干燥法脱水处理,两种冷季型草坪草匍匐翦股颖和高羊茅愈伤组织中18.5 kDa和28.7 kDa的热稳定蛋白表达量增加.暖季型草坪草狗牙根中18.5 kDa的热稳定蛋白表达量增加,而35 kDa的热稳定蛋白表达量明显减少.经脱水蛋白的Western Blot检测,脱水处理后3种草坪草中均有脱水蛋白大量积累,分子量从18.5到34.3 kDa不等.暖季型和冷季型草坪草在热稳定蛋白和脱水蛋白表达上存在的差异,可能与它们的耐旱机理及耐旱性强弱差异有关.

Calli of creeping bentgrass, tall rescue and bermudagrass were induced, and their rates of induction were 53.0±8.7%, 60.7±7.9%, and 14.7±4.0%, respectively, the water contents of callus were 91.4±0.3%, 94.1±0.4%, and 93.4±0.2%, respectively. After air-drying dehydration treatment, the expression level of heat-stable proteins with molecular weight of 18.5 and 28.7 kDa in cool-season turf grass calli was increased. The heat-stable proteins with molecular weight of 18.5 kDa in warm-season turf grass bermudagrass was also accumulated, while only one heat-stable protein with molecular weight of 35 kDa was obviously reduced. Results from immunological detection of dehydrin showed that the amount of dehydrins in three turf grass, with the molecular weight from 18.5 kDa to 34. 3 kDa, increased significantly. The differences in accumulation of heat-stable proteins and dehydrins might be related to difference mechanisms of drought tolerance between warm-season and cool-season turf grass.